Spectrum-effect correlation analysis of traditional Tibetan medicine "Morina nepalensis" on nitric oxide production inhibition / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2882-2885, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-238627
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC-ELSD fingerprint of the whole herbs of Morina nepalensis and perform the correlation analysis of chemical components of the herb and nitric oxide (NO) production inhibition.</p><p><b>METHOD</b>HPLC-ELSD assay was performed to evaluate 10 batches of M. nepalensis herbs. The chromatographic conditions were as following Eclipse XDB C18 column (4.6 mm x 150 mm, 5 microm), water (A) and acetonitrile (B) as a gradient mobile phases, flow rate 1.0 mL x min(-1), and column temperature at 35 degress C. Evaporative light-detection conditions atomization temperature at 104 degrees C, the flow rate of N2 2.8 L x min(-1) and 10 microL sample injection. Chromatographic fingerprint was developed, and the inhibition activity of production of NO in lipopolysaccharide-induced macrophages was also analyzed. The similarity and correlation analysis between the HPLC-ELSD fingerprints and NO production inhibition were carried out by PLS method.</p><p><b>RESULT</b>The common mode for M. nepalensis herb fingerprint was established, including 15 common characteristic peaks. Among them, 7 peaks were positively correlated with the NO production inhibition. According to the assessment on the similarity of 10 batches of samples, a similarity of over 0.90 were shown in HPLC-ELSD fingerprint and all samples were separated into two groups.</p><p><b>CONCLUSION</b>This method can be used to assess the quality of M. nepalensis, which provides a reliable method for scientific assessment and quality control.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Cell Line
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Chemistry
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Chromatography, High Pressure Liquid
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Caprifoliaceae
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Medicine, Tibetan Traditional
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Allergy and Immunology
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Macrophages
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Anti-Inflammatory Agents
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Document type:
Article