Differential expression of miRNAs in myocardial tissues of rats with lipopolysaccharide- induced endotoxemia / 南方医科大学学报
Journal of Southern Medical University
; (12): 213-217, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-239210
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the differential miRNA expression profile in the myocardium of rats with lipopolysaccharide (LPS)-induced endotoxemia and explore the role of miRNA in endotoxin-induced myocardial injury.</p><p><b>METHODS</b>Twenty male SD rats received intraperitoneal injection of 10 mg/kg LPS (n=10) or an equivalent amount of saline solution (n=10). At 24 h after LPS injection, the rats were sacrificed to detect myocardial expressions of TLR4, TNF-α and IL-1β using real-time PCR and for observing myocardial ultrastructures under transmission electron microscopy. The differentially expressed miRNA in the myocardium were detected using a miRNA array, and the common differentially expressed miRNAs were selected for verifying their actual expressions using real-time PCR.</p><p><b>RESULTS</b>TLR4, TNF-α and IL-1β were over-activated in the myocardium of LPS-treated rats, in which mitochondria swelling, structural damaged and cytoplasmic vacuoles were observed. In LPS-challenged rats, miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, and miR-877 were found up-regulated significantly, whereas miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, and miR-3585-5p down-regulated in the myocardium.</p><p><b>CONCLUSION</b>Significant differential expression of the miRNAs occurs in the myocardium of LPS-treated rats, suggesting their involvement in endotoxin-induced myocardial injury.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Up-Regulation
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Lipopolysaccharides
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Tumor Necrosis Factor-alpha
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Rats, Sprague-Dawley
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Endotoxemia
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MicroRNAs
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Toll-Like Receptor 4
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Interleukin-1beta
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Real-Time Polymerase Chain Reaction
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Injections, Intraperitoneal
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Document type:
Article