Study on the extraction recovery of ginsenoside Re in plasma by different solid-phase cartridges / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To optimize the solid-phase extraction method by comparison of the extraction recovery of ginsenoside Re plasma samples.</p><p><b>METHOD</b>After extracted by different solid-phase cartridges with water, acetonitrile, and different content methanol elution, the plasma samples were analyzed on an Zorbax SB-C18 column with acetonitrile-water gradient elution. From the recovery achieved, the best solid phase cartridge was found.</p><p><b>RESULT</b>This method consists of using 40% methanol as the wash solvent, and 80% methanol for the elution. Among the three kinds of solid-phase being tested, Waters Oasis HLB cartridge was found to be the best one.</p><p><b>CONCLUSION</b>The average extraction recovery of the Waters Oasis HLB cartridges was between 103%-113%, it can be used in the analysis of ginsenoside Re in plasma samples.</p>