An XcmⅠ-generated T vector and its applications in synthetic biology / 生物工程学报
Chinese Journal of Biotechnology
; (12): 956-965, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-242284
Responsible library:
WPRO
ABSTRACT
For more economical and efficient DNA clonging, pFL-XS-T, a Biobrick-T vector was constructed based on pMD18-T vector, carrying clonging regions of XbaⅠ-XcmⅠ-XcmⅠ-SpeⅠ. The results revealed that PCR products could be conveniently inserted into pFL-XS-T vevtor digested by XcmⅠby means of TA cloning. The positive frequency of recombination can meet the experimental requirements and all the plasmids obtained meet Biobrick standard. Moreover, the pFL-XS-T is compatible with other Biobrick parts, and serves as a vector for functional DNA fragments screening.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
DNA
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
Synthetic Biology
/
Genetic Vectors
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2016
Document type:
Article