Effect of fengshiqing on interleukin-4, gamma-interferon and chemotactic factor in rats with collagen type II / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 1114-1116, 2009.
Article
in Zh
| WPRIM
| ID: wpr-242346
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the regulatory effect of fengshiqing (FSQ) on interleukin 4 (IL-4), gamma-interferon (gamma-IFN), macrophage inflammatory protein 1alpha (MIP-1alpha) and collagen type II antibody (C II Ab) in serum and supernatant of synovial cell culture of rat with rheumatoid arthritis (RA), to explore the mechanism of action of clearing-heat and activating blood method for treatment of RA.</p><p><b>METHODS</b>RA rat model was induced by C II Ab combined with Freund's complete adjuvant, and the levels of IL-4, gamma-IFN, MIP-1alpha and C II Ab in serum and supernatant of synovial cell were detected by ELISA.</p><p><b>RESULTS</b>As compared with the normal group, serum level of C II Ab in the model group was significantly higher (P < 0.01), serum and supernatant contents of IL-4 on the 14th and 28th day of modeling were lower and those of gamma-IFN and MIP-1alpha were higher, the difference showed statistical significance (P < 0.05 or P < 0.01). After being treated with FSQ and IL-4 contents in serum and supernatant as well as MIP-1alpha in supernatant restored on the 14th day (P < 0.05 or P < 0.01), while all the indexes restored on the 28th day.</p><p><b>CONCLUSION</b>FSQ could evidently up-regulate the level of IL-4 and down-regulate that of MIP-1alpha in serum and local synovial membrane in RA rats, and shows a suppressive trend of gamma-IFN, so as to maintain the Th1/Th2 equilibrium, suppress the cellular and humoral immune response in the local synovial membrane, and alleviate the chronic changes of arthritis, synovitis and vasculitis.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Arthritis, Rheumatoid
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Drugs, Chinese Herbal
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Interleukin-4
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Interferon-gamma
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Rats, Wistar
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Collagen Type II
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Chemokine CCL3
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Th1-Th2 Balance
/
Metabolism
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2009
Document type:
Article