Distribution of Bifidobacterium in oral cavities of children and the relations with caries / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 618-621, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-242936
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore a selected-media of Bifidobacterium from oral cavity, to detect the distribution of Bifidobacterium in different sites of children and primarily investigate the relationship between oral Bifidobacterium and early childhood caries.</p><p><b>METHODS</b>70 children aged from 3 to 5-year-old were selected, 30 children were caries-free and 40 were severe early childhood caries (S-ECC). Saliva was collected and plaque samples from the 30 healthy subjects were pooled. For S-ECC group, plaques were collected separately from four different sites as follows Saliva, surfaces of intact enamel, surfaces of white spot-lesions, and deep dentin-lesions. Samples would be grown in the selected-media, and the whole DNA of bacteria was extracted. Polymerase chain reaction was performed with specific primers and the results were analyzed by the electrophoresis.</p><p><b>RESULTS</b>Bifidobacterium were detected 0 in the caries-free children, while 47.5% in the S-ECC group. There was significant difference between two groups (P < 0.05) and there was no difference between different sites of teeth in S-ECC group (P > 0.05). 27.5% Bifidobacterium were detected in saliva, 27.5% on surfaces of intact enamel, 20.0% on surfaces of white spot-lesions and 22.5% in deep dentin-lesions. 10% Bifidobacterium dentium were detected in saliva, 7.5% on surfaces of intact enamel, 7.5% on surfaces of white spot-lesions and 10.0% in deep dentin-lesions.</p><p><b>CONCLUSION</b>One type of modified selected media of Bifidobacterium in oral cavity was explored. Bifidobacterium may be related to the occurrence of the S-ECC and has nothing to do with different sites of teeth in children.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Saliva
/
Bacteria
/
Tooth
/
Bifidobacterium
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Polymerase Chain Reaction
/
Dental Caries
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Dental Enamel
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Dental Plaque
/
Dentin
Limits:
Child
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Child, preschool
/
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2009
Document type:
Article