Effect of bortezomib on sensitization of HL-60 cells to TRAIL / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 617-620, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-243301
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore whether bortezomib can sensitize HL-60 cells to TNF-related apoptosis-inducing ligand (TRAIL) and to investigate its possible mechanism. The HL-60 cells were treated by different concentrations of TRAIL combined with subtoxic concentration of bortezomib. The proliferative inhibition of treated HL-60 cell was analysed by MTT assay. The cell apoptosis was determined by flow cytometry with Annexin V/PI double staining and the expression of caspase-8 was detected by Western blot. The results showed that the subtoxic concentration of bortezomib combined with 10 ng/ml of TRAIL enhanced apoptosis of HL-60 cells, as compared with TRAIL used alone; the expression of caspase-8 increased correspondingly. It is concluded that subtoxic concentration of bortezomib can sensitize HL-60 cells to TRAIL and its mechanism may be related to upregulation of caspase-8 expression.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyrazines
/
Boronic Acids
/
Apoptosis
/
HL-60 Cells
/
Caspase 8
/
TNF-Related Apoptosis-Inducing Ligand
/
Bortezomib
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2010
Document type:
Article