Build of focal cerebral ischemia model in different varieties of mice with modification monofilament / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3367-3370, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-244562
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a general method of focal cerebral ischemia model in different varieties of mice.</p><p><b>METHOD</b>Each group of healthy adult KM and C57BL/6 mice were randomly divided into control group (n = 10) and MCAO group (n = 10). The mice in MCAO group were applied in the preparation of the MCAO model by intraluminal occlusion using monofilament. Twenty-four hours after operation,the neurologic function was evaluated,middle cerebral artery blood flow was monitored and the infarction volume was calculated by TTC staining, to evaluate the reliability of the model.</p><p><b>RESULT</b>In the MCAO group, the base value of the cerebral blood flow down of KM and C57BL/6 mice respectively was (81.65 ± 4.59)%, (83.68 ± 6.25)%. The neurological deficit score respectively was (2.30 ± 0.82), (2.50 ± 0.80). TTC staining can clearly show the infarction area, and relatively stable, 24 hours of the survival rate of KM and C57BL/6 mice were 100% and 80% respectively.</p><p><b>CONCLUSION</b>The key link is the optimization and improvement of monofilament, temperature, anesthesia and so on. The modified intraluminal occlusion of MCAO using monofilament is a kind of reliable and simple method to establish experimental cerebral ischemia model in mice.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Species Specificity
/
General Surgery
/
Blood Flow Velocity
/
Brain
/
Brain Ischemia
/
Cerebrovascular Circulation
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Middle Cerebral Artery
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Infarction, Middle Cerebral Artery
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Disease Models, Animal
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Document type:
Article