Protective effect of Noningkang Granule on brain in intracerebral hemorrhagic rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 814-818, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-245635
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effect and mechanism of Naoningkang Granule (NG), a Chinese medicinal preparation formulated for clearing heat and detoxication, on brain tissue in intracerebral hemorrhagic (ICH) rats.</p><p><b>METHODS</b>Rats were randomly divided into 5 groups the sham operated group, the model group and the high-, medium- and low-dose NG groups. Collagenase VII was injected into caudate nucleus to induce rat model of ICH, corresponding dosage of NG was started to give to the 3 NG groups by gastrogavage 2 h after modeling, and saline of equal volume was given to the other 2 groups instead. The brain tissue of rats was taken in batches at the 3rd and 7th day for pathomorphological observation using HE stain, and detection of thrombin receptor-1 (PAR-1) expression and nerve cell apoptosis in the peripheral tissue of hemorrhagic brain with immunohistochemistry and TUNEL assay, as well as for measurement of water content in brain tissue by wet-to-dry weight method.</p><p><b>RESULTS</b>PAR-1 expression elevated in the model rats. As compared with the model group, the pathomorphological changes significantly improved, PAR-1 expression decreased, apoptotic cells re-duced and brain edema alleviated in the 3 NG groups.</p><p><b>CONCLUSION</b>Overexpression of PAR-1 in the brain tissue might mediate the nerve cell apoptosis and brain edema in ICH rats. The mechanism of NG in protecting hemor-rhagic brain tissue might be related with its actions in inhibiting the post-cerebral high PAR-1 expression to re-duce cell apoptosis and relieve brain edema.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Cardiovascular Disease
/
Cerebrovascular Disease
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Brain
/
Brain Edema
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Drugs, Chinese Herbal
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Random Allocation
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Cerebral Hemorrhage
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Rats, Wistar
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Apoptosis
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Neuroprotective Agents
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Receptor, PAR-1
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2007
Document type:
Article