Effects and mechanism of lutein on apoptosis of esophageal carcinoma EC9706 cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 332-354, 2007.
Article
in Zh
| WPRIM
| ID: wpr-245979
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of lutein on apoptosis and its mechanism.</p><p><b>METHOD</b>The cells of human esophageal carcinoma EC9706 were grown in RPMI medium containing 10% bovine serum and were treated with lutein at 100 microg x mL(-1) concentration. Flow cytometry was employed to investigate the effects of lutein on cell apoptosis of EC9706 cells. Histochemistry was performed to determine apoptosis-related protein expresion.</p><p><b>RESULT</b>Flow cytometry analyses revealed that lutein increased EC9706 cell apoptosis ratio when treated with lutein 100 microg x mL(-1) at 96 h. Lutein decreased the expression of Bcl-2 protein and increased the expression of Bax protein in EC9706 cells.</p><p><b>CONCLUSION</b>Lutein could inhibit mitosis and stimulate apoptosis of EC9706 cells. The apoptotic effect may result from the down-regulation of expression of Bcl-2 and up-regulation expression of Bax.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Lutein
/
Esophageal Neoplasms
/
Carcinoma, Squamous Cell
/
Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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Cell Line, Tumor
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Bcl-2-Associated X Protein
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Document type:
Article