Effects of among compositions of Herba Ephedrae decoction on genic xpression of 5-lipoxygenase activating protein, IL-4 and leukotriene C4 in asthmatic mice / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 246-249, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-245998
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the regularity of recipe composition by observing inhibitory effects on the genic expression of 5-lipoxygenase activating protein, IL-4 and the leukotriene C4 in asthmatic mice.</p><p><b>METHOD</b>The mice were challenged with OVA and administered ig with the Herba Ephedrae decoction (HED), separated compositions (2500 mg x kg(-1), calculated by Herba Ephedrae) and dexamethasone (2 mg x kg(-1)) respectively once daily for seven days. The real-time fluorescence quantitative PCR method was employed to measure the contents of FLAP mRNA and IL-4 mRNA expressions in lung and the ELISA method was used to determine the content of LTC4 in the washing solution of pulmonary alveolus and bronchi.</p><p><b>RESULT</b>In the lung of asthma mice, the expressions of FLAP and IL-4 and the content of LTC4 were significantly augmented compared with the control group. The HED and the separated compositions could suppress the expressions of FLAP and IL-4 and LTC4 release to a great extent in mice.</p><p><b>CONCLUSION</b>The HED had the remarkable effects of antianaphylaxis asthma and the original formula HED worked best. These results confirmed the rationality and scientific level of HED.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
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Asthma
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RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
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Bronchoalveolar Lavage Fluid
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Drugs, Chinese Herbal
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Carrier Proteins
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Random Allocation
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Chemistry
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Document type:
Article