Effects of carbon source and nitrogen source on callus growth and flavonoid content in Glycyrrhiza uralensis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1857-1859, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-246063
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of carbon source and nitrogen source on callus growth, and flavonoid content in Glycyrrhiza uralensis.</p><p><b>METHOD</b>Induction and culture of callus were conducted in the media of different concentrations of sucrose, and fructose and ratio of NH4+/NO3- ,and flavonoid content was measured by HPLC.</p><p><b>RESULT</b>The results showed that fructose was superior to sucrose for callus growth and flavonoid formation, and the optimum concentration was 2%. The flavonoids content was 2 times higher than that of sucrose as carbon sources. In the range of 2% -6% of fructose concentration, the flavonoid content was decreased along with the concentration of fructose, but the licochalcone was increased 5-fold. The highest flavonoid content of 151.47 microg x g(-1) was obtained when the ratio of NH4+/NO3- in the medium was 1/2. NH; inhibited the callus growth and flavonoid formation of G. uralensis.</p><p><b>CONCLUSION</b>Fructose as carbon source was superior to sucrose for callus growth and flavonoid formation, and NO3- was favorable to the callus growth and flavonoids accumulation.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
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Sucrose
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Flavonoids
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Nitrogen Compounds
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Culture Media
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Glycyrrhiza uralensis
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Tissue Culture Techniques
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Disaccharides
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Fructose
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Document type:
Article