Inhibitory Effect of Serum Containing Fuzheng Jiedu Decoction on the Leukemia K562/A02 Multi-drug Resistance Cells and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1024-1028, 2016.
Article
in Zh
| WPRIM
| ID: wpr-246823
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effect of serum containing Fuzheng Jiedu decoction on leukemia multi-drug-resistance K562/A02 cells and its possible mechanism.</p><p><b>METHODS</b>The MTT method was used to detect the inhibitory rate of K562/AO2 cells treated with serum containing Fuzheng Jiedu decoction; the flow cytometry was used to detect the inhibitory effect of serum containing medicin on growth of K562/AO2 cells and P-gp expression; the Q-PCR was used to assay the BCL-2 mRNA expression; the Western blot was used to detect the BCL-2 protein expression.</p><p><b>RESULTS</b>MTT cytotoxic test showed serum containing Fuzheng Jiedu decoction could inhibit K562/A02 cell growth, and the inhibitory rate increased with the increase of drug concentration; the flow cytometry showed that the serum containing Fuzheng Jiedu decoction could promote K562/A02 cell apoptosis in a concentration-dependent manner. qPCR and Western blot showed that serum containing Fuzheng Jiedu decoction could down-regulate the protein expression of BCL-2. Fuzheng Jiedu decoction could reduce the protein expression of P-gp on the K562/A02 cell membrane.</p><p><b>CONCLUSION</b>serum containing Fuzheng Jiedu decoction can promote K562/A02 cell apoptosis, its mechanism of inducing apoptosis may be related with the inhibition of BCL-2 and P-gp protein expression.</p>
Full text:
1
Database:
WPRIM
Main subject:
Drugs, Chinese Herbal
/
Leukemia
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Apoptosis
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Drug Resistance, Multiple
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Drug Resistance, Neoplasm
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K562 Cells
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Cell Proliferation
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2016
Document type:
Article