The development of a fluorescence polarization immunoassay for aflatoxin detection / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 126-129, 2014.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-247074
Responsible library:
WPRO
ABSTRACT
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 min. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Fluorescence Polarization Immunoassay
/
Aflatoxins
Type of study:
Diagnostic study
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2014
Document type:
Article