Effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase expression during myocardial ischemia-reperfusion in rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 535-539, 2011.
Article
in Zh
| WPRIM
| ID: wpr-247218
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase (GST) expression during myocardial ischemia-reperfusion in rats.</p><p><b>METHODS</b>Sprague-Dawley male rats were randomly divided into 4 groups (n= 10 in each): Group S (sham operation group), Group IR (ischemia/reperfusion group), Group H (IR+ NaHS 0.05 mg/kg iv, 24 h before ischemia) and Groups D receiving IR+NaHS 24 h before ischemia and 5-hydroxydecanoate (5-HD)15 min before ischemia. Animals in groups IR, H and D were subjected to ischemia by 30 min of coronary artery occlusion followed by 2 h of reperfusion. At the end of the reperfusion, myocardial infarct size (IS) was examined. Glutathione S-transferase (GST) was measured by Western blotting. The myocardial ultrastructures were observed under the electron microscopy.</p><p><b>RESULTS</b>The IS was significantly smaller in Group H than that in Group IR [(25.40 ± 3.54)% compared with (38.27 ± 5.64)%, P<0.05]. The GST expression in myocardium was significantly higher in Group H than that in Group IR. Microscopic examination showed less myocardial damage in Group H than in Group IR. The protective effects of delayed preconditioning by hydrogen sulfide was prevented by 5-HD pre-treatment.</p><p><b>CONCLUSION</b>The hydrogen sulfide-induced delayed preconditioning attenuates myocardial IR injury possibly through up-regulating glutathione S-transferase expression in rats.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Therapeutics
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Myocardial Reperfusion Injury
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Rats, Sprague-Dawley
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Ischemic Preconditioning, Myocardial
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Therapeutic Uses
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Disease Models, Animal
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Glutathione Transferase
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Hydrogen Sulfide
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2011
Document type:
Article