Optimization of a HPLC determination method for Evodia rutaecarpa / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 478-480, 2011.
Article
in Zh
| WPRIM
| ID: wpr-247453
Responsible library:
WPRO
ABSTRACT
A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
Full text:
1
Database:
WPRIM
Main subject:
Quinazolines
/
Plant Extracts
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Chemistry
/
Chromatography, High Pressure Liquid
/
Indole Alkaloids
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Evodia
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Limonins
/
Methods
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Document type:
Article