Expression of human anti-apoptotic gene survivin and its splice in normal human gastric tissue and gastric cancer / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 75-76, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-248493
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the expression of human anti-apoptotic gene survivin (SVV) in normal human gastric tissues and gastric cancer.</p><p><b>METHODS</b>SVV cDNA clone was obtained from human gastric cancer tissues by virtue of RT-PCR, using Dig-marked cRNA probe in situ hybridization to analyze its expression in normal human gastric tissues and gastric cancer.</p><p><b>RESULTS</b>Two SVV cDNA clones, SVV-S4A and SVV-S1B were obtained. The sequence of the former is identical to that of the well-known SVV cDNA; however, in the sequence of the latter, the third exon was missed, i.e., there are only two exons in SVV-S1B. In situ hybridization showed that SVV-S4A is mainly expressed in gastric cancer tissues whereas SVV-S1B is mostly expressed in normal gastric tissues.</p><p><b>CONCLUSION</b>There is difference between SVV-S4A and SVV-S1B in respect to their characteristics of expression in gastric cancer and normal gastric tissues.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
Molecular Sequence Data
/
Base Sequence
/
Gene Expression Regulation, Neoplastic
/
Chemistry
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Alternative Splicing
/
DNA, Complementary
/
Inhibitor of Apoptosis Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2003
Document type:
Article