Effect of thymic stromal lymphopoietin on human bronchial epithelial permeability / 南方医科大学学报
Journal of Southern Medical University
; (12): 802-806, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-249355
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of thymic stromal lymphopoietin (TSLP) on the permeablily of monolayer bronchial epithelial cells in vitro.</p><p><b>METHODS</b>Cultured human bronchial epithelial cell line 16HBE was exposed to 0.1 or 1 ng/ml TSLP for 0, 0.5, 6, 12, or 24 h, and the epithelial monolayer permeability was assessed by measuring transepithelial electrical resistance (TER), permeability to FITC-labeled dextran (FITC-DX) and expression of E-cadherin.</p><p><b>RESULTS</b>Compared with the control cells group, 16HBE cell monolayer showed significantly increased TER (P<0.001) and decreased FITC-DX fluorescence in the lower chamber (P<0.05) following exposure to 0.1 and 1 ng/ml TSLP, but these changes were not dose-dependent. Exposure to 0.1 ng/ml TSLP resulted in significantly increased expression of E-cadherin. The 16HBE monolayer exposed to 0.1 ng/ml TSLP for 24 h showed the most obvious increase of TER and E-cadherin expression (P<0.05); FITC-DX fluorescence level was markedly decreased after TSLP exposure for 12 h and 24 h (P<0.05), and the effect was more obvious in 12 h group.</p><p><b>CONCLUSION</b>TSLP can protect the barrier function of normal bronchial epithelial cells in vitro.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Pharmacology
/
Bronchi
/
Cadherins
/
Cell Line
/
Cytokines
/
Cell Biology
/
Epithelial Cells
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2014
Document type:
Article