Effects of Genistein on the proliferation and expression of survivin in salivary adenoid cystic carcinoma cell line SACC-83 / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 97-99, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-249744
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-proliferation effect of tyrosine protein kinase inhibitor, Genistein, on human salivary adenoid cystic carcinoma cell line SACC-83, and its effect on Survivin expression.</p><p><b>METHODS</b>SACC-83 cells were treated with different concentration Genistein for different time, cell survival rate was calculated with MTT assay, apoptosis was detected with flow cytometry, the expression of Survivin was quantitatively analyzed by Western blotting and FluorChem V2.0 software.</p><p><b>RESULTS</b>When treated with Genistein of certain concentration for certain time, SACC-83 cell growth was significantly inhibited. With the increase of concentration and elongation of acting time, the inhibitory effects increase. Treated with 220 micromol/L Genistein for 72 hours, SACC-83 cell growth was significantly inhibited, cell apoptosis was induced (P < 0.01), and the expression of Survivin decreased.</p><p><b>CONCLUSION</b>Genistein inhibits the growth of human salivary adenoid cystic carcinoma cell line SACC-83, and induces cell apoptosis; the decrease of Survivin expression may be one of the mechanisms of Genistein inducing apoptosis.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Mouth and Oropharynx Cancers
/
Other Respiratory Diseases
Database:
WPRIM (Western Pacific)
Main subject:
Salivary Gland Neoplasms
/
Cell Line
/
Apoptosis
/
Carcinoma, Adenoid Cystic
/
Genistein
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2007
Document type:
Article