Induction of apoptosis by recombinant soluble human TRAIL in Jurkat cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 470-477, 2007.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-249823
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic potential of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells.</p><p><b>METHODS</b>Expression of TRAIL receptors (DR4 and DR5) was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation (DNA ladder) and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence.</p><p><b>RESULTS</b>TRAIL inhibited the proliferation and induced internucleosomal DNA fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential.</p><p><b>CONCLUSION</b>Recombinant soluble TRAIL can be used as a therapy for cancer.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Solubility
/
Recombinant Proteins
/
Base Sequence
/
Apoptosis
/
DNA Primers
/
Jurkat Cells
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Reverse Transcriptase Polymerase Chain Reaction
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Electrophoresis, Agar Gel
/
TNF-Related Apoptosis-Inducing Ligand
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2007
Document type:
Article