Possible role of DNA polymerase beta in protecting human bronchial epithelial cells against cytotoxicity of hydroquinone / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 171-177, 2007.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-249870
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the toxicological mechanism of hydroquinone in human bronchial epithelial cells and to investigate whether DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone.</p><p><b>METHODS</b>DNA polymerase beta knock-down cell line was established via RNA interference as an experimental group. Normal human bronchial epithelial cells and cells transfected with the empty vector of pEGFP-C1 were used as controls. Cells were treated with different concentrations of hydroquinone (ranged from 10 micromol/L to 120 micromol/L) for 4 hours. MTT assay and Comet assay [single-cell gel electrophoresis (SCGE)] were performed respectively to detect the toxicity of hydroquinone.</p><p><b>RESULTS</b>MTT assay showed that DNA polymerase beta knock-down cells treated with different concentrations of hydroquinone had a lower absorbance value at 490 nm than the control cells in a dose-dependant manner. Comet assay revealed that different concentrations of hydroquinone caused more severe DNA damage in DNA polymerase beta knock-down cell line than in control cells and there was no significant difference in the two control groups.</p><p><b>CONCLUSIONS</b>Hydroquinone has significant toxicity to human bronchial epithelial cells and causes DNA damage. DNA polymerase beta knock-down cell line appears more sensitive to hydroquinone than the control cells. The results suggest that DNA polymerase beta is involved in protecting cells from damage caused by hydroquinone.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
DNA Damage
/
Bronchi
/
Cells, Cultured
/
DNA Polymerase beta
/
Comet Assay
/
Cell Biology
/
Cytotoxins
/
RNA Interference
/
Epithelial Cells
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2007
Document type:
Article