Study of recombinant stem cell factor / 生物工程学报
Chinese Journal of Biotechnology
; (12): 698-704, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-250005
Responsible library:
WPRO
ABSTRACT
Stem cell factor is an important hematopoietic growth factor. In this study, the human stem cell factor was produced by recombinant E. coli, and the structure and biological activity of the recombinant stem cell factor(rhSCF) was studied. It was indicated that the rhSCF was a uncovalent dimer in phosphate buffer,and had the correct mass spectra, mass peptides spectra, composition of amino acid, N-terminal sequernce, C-terminal sequence and intrachain disulfide linkages, rhSCF alone or synergy with rhG-CSF could mobilze hematopoietic progenitors to blood in monkey.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Recombinant Proteins
/
Hematopoietic Stem Cells
/
Cell Differentiation
/
Cells, Cultured
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Spectrometry, Mass, Fast Atom Bombardment
/
Haplorhini
/
Stem Cell Factor
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2003
Document type:
Article