Umbilical cord blood-derived mesenchymal stem cells ameliorate graft-versus-host disease following allogeneic hematopoietic stem cell transplantation through multiple immunoregulations / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 477-484, 2015.
Article
in En
| WPRIM
| ID: wpr-250392
Responsible library:
WPRO
ABSTRACT
Although mesenchymal stem cells (MSCs) are increasingly used to treat graft-versus-host disease (GVHD), their immune regulatory mechanism in the process is elusive. The present study aimed to investigate the curative effect of third-party umbilical cord blood-derived human MSCs (UCB-hMSCs) on GVHD patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and their immune regulatory mechanism. Twenty-four refractory GVHD patients after allo-HSCT were treated with UCB-hMSCs. Immune cells including T lymphocyte subsets, NK cells, Treg cells and dendritic cells (DCs) and cytokines including interleukin-17 (IL-17) and tumor necrosis factor-alpha (TNF-α) were monitored before and after MSCs transfusion. The results showed that the symptoms of GVHD were alleviated significantly without increased relapse of primary disease and transplant-related complications after MSCs transfusion. The number of CD3(+), CD3(+)CD4(+) and CD3(+)CD8(+) cells decreased significantly, and that of NK cells remained unchanged, whereas the number of CD4(+) and CD8(+) Tregs increased and reached a peak at 4 weeks; the number of mature DCs, and the levels of TNF-α and IL-17 decreased and reached a trough at 2 weeks. It was concluded that MSCs ameliorate GVHD and spare GVL effect via immunoregulations.
Full text:
1
Database:
WPRIM
Main subject:
Therapeutics
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Transplantation, Homologous
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Dendritic Cells
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Killer Cells, Natural
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Cytokines
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T-Lymphocyte Subsets
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Hematopoietic Stem Cell Transplantation
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Cord Blood Stem Cell Transplantation
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Allergy and Immunology
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Immunomodulation
Limits:
Adolescent
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Adult
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Female
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Humans
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Male
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2015
Document type:
Article