Preparation of baicalein using thermophilic and sugar-tolerant beta-glucosidase / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4616-4622, 2015.
Article
in Zh
| WPRIM
| ID: wpr-250444
Responsible library:
WPRO
ABSTRACT
The reaction conditions of baicalin hydrolyzed into baicalein by a kind of thermophilic and sugar-tolerant beta-glucosidase were studied in this paper. The beta-glucosidase could catalyze baicalin into baicalein well in the acetic acid-sodium acetate buffer. The optimal enzyme activity was at 85 degrees C and pH 5.5. The enzyme was stable at the temperature less than 85 degrees C and pH range of 5-7.5. The maximum reaction rate V. and michaelis constant K. were 0.41 mmol x L(-1) x min(-1) and 3.31 mmol x L(-1) respectively. Different metal ions had different effects on the activity of enzyme. Na+ existing in acetic acid-sodium acetate buffer had an activation effect on enzyme. The enzyme activity was enhanced by the concentrations of glucose below 0.6 mol x L(-1), and was gradually inhibited when monosaccharide concentration was over 0.6 mol x L(-1). When the monosaccharide concentration reached 1.2 mol x L(-1), the inhibition rate of enzyme activity was about 50%, which showed good glucose tolerance. The good reaction conditions through the experiment have been determined as follows, the substrate: enzyme dose was 1 g: 0.2 mL, acetic acid-sodium acetate buffer pH 5.5, reaction temperature 85 degrees C, reaction time 10 h, and the enzymatic hydrolyzation ratio could reach 97%.
Full text:
1
Database:
WPRIM
Main subject:
Flavonoids
/
Enzyme Stability
/
Kinetics
/
Chemistry
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Beta-Glucosidase
/
Flavanones
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Biocatalysis
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Glucose
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Hot Temperature
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Hydrolysis
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Document type:
Article