LC-MS/MS method for simultaneous quantification of lopinavir and ritonavir in human plasma / 药学学报
Acta Pharmaceutica Sinica
; (12): 279-282, 2010.
Article
in Zh
| WPRIM
| ID: wpr-250629
Responsible library:
WPRO
ABSTRACT
A liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of lopinavir and ritonavir in human plasma. Analytes were separated from plasma by a combination of alkalinized protein precipitation and liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Agilent ZORBAX Eclipse XDB-C18 column with the mobile phase consisted of methanol-0.1% formic acid in water (80:20). A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 629.6 --> 155.2, m/z 721.4 --> 268.2, and m/z 515.2 --> 276.2 for lopinavir, ritonavir and telmisartan (internal standard), respectively. The method showed a good linearity in a concentration range of 62.5 - 10000 ng mL(-1) for lopinavir, and 12.5 - 2000 ng mL(-1) for ritonavir. The lower limits of quantification were 15 pg mL(-1) and 8 pg mL(-1) for lopinavir and ritonavir, respectively. The intra- and inter-day precision was less than 15% and the absolute recovery was above 75%. This method was selective and rapid, sensitive for investigating blood drug concentrations in clinics.
Full text:
1
Database:
WPRIM
Main subject:
Blood
/
HIV Infections
/
Chromatography, Liquid
/
Sensitivity and Specificity
/
Ritonavir
/
Tandem Mass Spectrometry
/
Lopinavir
/
Methods
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2010
Document type:
Article