Downregulation of p38 MAPK involved in inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 666-671, 2013.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-251412
Responsible library:
WPRO
ABSTRACT
Curcumin, as a main pharmacological component in the traditional Chinese medicine-turmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the possible underlying signaling pathway which was involved in the inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin. Rat mesangial cells in vitro were incubated with low-density lipoprotein (LDL) and different concentrations of curcumin (0, 6.25, 12.5, 25.0 μmol/L) or p38 MAPK inhibitor, SB203580 (10 μmol/L). Under LDL incubation, mesangial cells proliferated, the expression of MMP-2 mRNA and protein was decreased, the expression of COX-2 mRNA and protein was increased, reactive oxygen species (ROS) generation was increased and p38 MAPK was activated significantly (P<0.05). When LDL-induced cells were treated with curcumin in the concentration of 12.5 or 25.0 μmol/L, LDL-induced proliferation of mesangial cells was suppressed, the expression of MMP-2 mRNA and protein increased, the expression of COX-2 mRNA and protein downregulated, the production of ROS inhibited and p38 MAPK inactivated (P<0.05). In conclusion, curcumin can inhibit the LDL-induced proliferation of mesangial cells and up-regulate the expression of MMP-2, which may be related with the inhibitory effect of curcumin on COX-2 expression, ROS production and p38 MAPK.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pyridines
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Down-Regulation
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Gene Expression
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Cells, Cultured
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Anti-Inflammatory Agents, Non-Steroidal
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Blotting, Western
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Reactive Oxygen Species
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2013
Document type:
Article