The role of amino acid sequence between 551 and 565 in the cytoplasmic domain of glycoprotein (GP) I b alpha in the regulation of the VWF binding to GP I b alpha / 中华血液学杂志
Chinese Journal of Hematology
; (12): 618-621, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-251513
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of the amino acids between 551 and 565 in the cytoplasmic domain of glycoprotein (GP) I b alpha in the VWF binding to GP I b alpha.</p><p><b>METHODS</b>The VWF binding to GP I b alpha induced by ristocetin was analyzed by flow cytometry, in three GP I b-IX-expressing Chinese hamster ovary (CHO) cell lines 1b9, delta 565 and delta 551, adhesion of above cells on VWF by flow chamber analysis at shear rate of 200 s(-1). The spread of GP I b-IX-expressing cells were stimulated with botrocetin on VWF-coated coverslips by confocal microscope.</p><p><b>RESULTS</b>The VWF binding to GP I b alpha was higher in delta 565 cells stimulated by ristocetin than in delta 551 or 1b9 cells. The number of delta 565 cells adhered on the VWF-coated-chamber was more than that of controls at shear rate of 200 s(-1). Moreover, the surface spreading areas of delta 565 cells were greater than that of the controls on VWF-coated coverslips.</p><p><b>CONCLUSIONS</b>The amino acids between 551 and 565 in the cytoplasmic domain of GP I b alpha regulates the VWF binding to GP I b alpha.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Von Willebrand Factor
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Platelet Adhesiveness
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Cricetulus
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Amino Acid Sequence
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CHO Cells
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Platelet Glycoprotein GPIb-IX Complex
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Genetics
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Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2011
Document type:
Article