Effect of bortezomib on the drug sensitivity of imatinib resistant K562/G01 cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 392-395, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-251943
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of bortezomib (BOR) on the drug sensitivity of imatinib-resistant chronic myeloid leukemia cell line K562/G01 cell and its mechanism.</p><p><b>METHODS</b>MTT assay was used to detect the inhibition effect of cell growth, flow cytometry to cell cycle, and real time-PCR to the expression of COX-2 and mdr1 mRNA.</p><p><b>RESULTS</b>Combination of 10 and 20 nmol/L BOR with imatinib could significantly enhance the sensitivity of K562/G01 to imatinib, the reverse factor was 1.83 and 2.72-fold respectively. Cell cycle arrested at G(2)/M phase could be observed by flow cytometry on BOR treatment. The over-expression of COX-2 and mdr1 could be down-regulated by BOR.</p><p><b>CONCLUSIONS</b>BOR can enhance the imatinib sensitivity of imatinib resistant K562/G01 cell. The mechanism may be related to cell cycle phase arrested at G2/M and down-regulation of COX-2 and mdr1 expression.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Piperazines
/
Pyrazines
/
Pyrimidines
/
Benzamides
/
Boronic Acids
/
Cell Cycle
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Neoplasm
/
ATP Binding Cassette Transporter, Subfamily B
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2011
Document type:
Article