Application of FICTION technique to the detection of genetic aberrations in multiple myeloma / 中华血液学杂志
Chinese Journal of Hematology
; (12): 226-230, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-251987
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the diagnostic value of FICTION (Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms) technique, combining immunofluorescence and fluorescence in situ hybridization (FISH), to detect genetic aberrations in multiple myeloma (MM).</p><p><b>METHODS</b>Bone marrow samples were collected from 18 MM and 2 plasma cell leukemia (PCL) patients. Probes targeting IgH and MMSET were prepared using a Nick Translation Kit from Bacterial artificial chromosome (BAC) clones. The immunophenotyping was achieved via the CD138 tyramide signal amplification (TSA)-mediated immunofluorescence, followed by FISH with the prepared probes \[t(4;14), t(11;14), t(14;16)\] and the commercial deletion probes (13q and p53) to detect common genetic aberrations in MM.</p><p><b>RESULTS</b>All the 20 samples were assayed with the probes mentioned above, and revealed 4 cases with t(4;14), 6 with t(11;14), 1 with t(14;16), 3 with p53 deletion; and 8 with 13q deletion. The remaining 4 cases had none of the 5 aberrations.</p><p><b>CONCLUSION</b>FICTION technique facilitates the detection of genetic abnormalities of MM in situ; enhances both efficiency and sensitivity of positive detection, thus, could be used as the screening test of molecular diagnosis of MM to guide coming-up risk-adapted therapy and evaluate prognosis.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Immunophenotyping
/
Fluorescent Antibody Technique
/
In Situ Hybridization, Fluorescence
/
Cytogenetics
/
Diagnosis
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Genetics
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Multiple Myeloma
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Aged, 80 and over
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Female
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Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2011
Document type:
Article