Application of orthogonal design in tissue culture of Dioscorea opposite / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose.</p><p><b>METHOD</b>stems were selected as explants. The effects of three kinds of factors were studied by orthogonal design method including sucrose, 6-BA, NAA on the buds and microtuber induction. The data were analyzed with range analysis and vadance analysis. RESULT AND CONDUSION The optimal media to induce many buds from stems were MS + 6-BA 1 mg x L(-1) + NAA 1 mg x L(-1) + sucrose 3%, the effect of the three factors was in sequence of sucrose >6-BA > NAA. The optimal media to induce microtuber from stems were MS+6-BA 1.5 mg x V1 +NAA 1.5 mg x L(-1) + sucrose 5%, the effect of the three factors was in sequence of sucrose >6-BA > NAA.</p>