The assay of transcriptional activation effect of TR3 and deletion mutation in the yeast two hybrid system / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 504-507, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-252727
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To assay the transcriptional activation effect of TR3 and it's deletion mutation in yeast two hybrid system.</p><p><b>METHODS</b>The total length of TR3 and TR3/delta1-690 gene was amplified by PCR method and cloned into pGBKT7 vector. Bait vector of pGBKT7-TR3 and pGBKT7-TR3/delta1-690 was transformed into AH109 competence yeast. Then self activation of the recombination vector was tested by assay the activity of beta-galactosidae.</p><p><b>RESULTS</b>The pGBKT7-TR3 and pGBKT7-TR3/AM 690 vector was successfully constructed. The filter paper containing beta-galactosidae didn't changed to blue showed that the reporter gene wasn't activationed.</p><p><b>CONCLUSION</b>TR3 and TR3/delta1-690 hadn't the activity of transcriptional activation.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Transcriptional Activation
/
Sequence Deletion
/
Two-Hybrid System Techniques
/
Nuclear Receptor Subfamily 4, Group A, Member 1
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2008
Document type:
Article