Effects of lactate on cell injury in primary cultures of rat cortical neurons during hypoxia/reoxygenation / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 434-438, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-252748
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To explore the effects of different concentrations of lactate on neuronal injury during hypoxia/reoxygenation (H/R) and its mechanism.</p><p><b>METHODS</b>Different concentrations of lactate (0, 0.5, 1.0, 2.0, 5.0 mmol/L) were added into medium after different duration of hypoxia, then reoxygenation for 24 h, cell survival rate and LDH release were assayed to determine neuronal damage, moreover, equal concentration of hydrochloric acid were used to mimic the changes of pH brought by lactate for investigating the mechanism of the effects of lactate on neuronal H/R injury.</p><p><b>RESULTS</b>Under normoxia and H/R 5.0 mmol/L lactate and hydrochloric acid induced or exacerbated neuronal injury. After 12 h and 24 h hypoxia exposure 1.0 mmol/L lactate was shown to be protective, 1.0 mmol/L hydrochloric acid had no effect on neuronal H/R damage.</p><p><b>CONCLUSION</b>Lactate of lower concentration was demonstrated to be neuroprotective during H/R, this protective effect was shown to be due to lactate anions. In contrast, higher concentration of lactate could induce or aggravate neuronal damage under normoxia and H/R, perhaps via the mechanism which involved the changes of pH.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Reperfusion Injury
/
Cell Hypoxia
/
Cells, Cultured
/
Cerebral Cortex
/
Rats, Wistar
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Neuroprotective Agents
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Lactic Acid
/
Cell Biology
/
Primary Cell Culture
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2008
Document type:
Article