The expression of Sonic Hedgehog during late bell stage by Western Blot and in situ hybridization / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 210-212, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-253743
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and biological effect of Shh during late bell stage by morphological and semi-quantitative analysis.</p><p><b>METHODS</b>Tooth germs were selected from new born Bal b/c mouse (P1, P2, P3, P5, P7). Semi-quality of Shh was measured by Western Blot and the expression place and strength of Shh were observed by in situ hybridization.</p><p><b>RESULTS</b>Shh was expressed in the ameloblast layer during late bell stage; the expression strength was high in secretive period and decreased with development; the active N-section was detectable before P3.</p><p><b>CONCLUSION</b>Shh expresses specially in the ameloblast layer in late bell stage, and expression quality is related to the function of ameloblasts.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Tooth Germ
/
In Vitro Techniques
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Gene Expression
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Trans-Activators
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Blotting, Western
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In Situ Hybridization
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Cell Biology
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Hedgehog Proteins
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Ameloblasts
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Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2003
Document type:
Article