Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 933-938, 2014.
Article
in Zh
| WPRIM
| ID: wpr-254165
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of endogenous leukemia inhibitory factor (LIF) in neonatal rats with periventricular leukomalacia (PVL).</p><p><b>METHODS</b>A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia (HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP.</p><p><b>RESULTS</b>At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group (P<0.01). In the PVL group, the LIF protein level on the third day after HI reached a peak and was higher than the other time points (P<0.01). The change of LIF mRNA expression showed the same tendency with LIF protein. The double staining immunofluorescence showed a co-expression of LIF and GFAP.</p><p><b>CONCLUSIONS</b>LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Physiology
/
Leukomalacia, Periventricular
/
RNA, Messenger
/
Rats, Wistar
/
Disease Models, Animal
/
Leukemia Inhibitory Factor
/
Genetics
/
Glial Fibrillary Acidic Protein
/
Animals, Newborn
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2014
Document type:
Article