Analysis of allele dropout at TH01 locus in paternity testing / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 622-625, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-254548
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze allele dropout at TH01 locus in paternity testing in order to determine the accurate genotype.</p><p><b>METHODS</b>To use a two STR loci genotyping system to verify an abnormal genotype for the TH01 locus with PCR using specific primers, cloning and DNA sequencing.</p><p><b>RESULTS</b>A rare allele at TH01 locus named 5.2, which was undetectable with PowerPlex 21 system, was detected with an Identifiler system.</p><p><b>CONCLUSION</b>Genetic variations may result in rare alleles and loci loss. To avoid misjudgment, laboratories should have a variety of methods for detecting loci loss.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Paternity
/
Molecular Sequence Data
/
Base Sequence
/
Genetic Testing
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Sequence Deletion
/
Tandem Repeat Sequences
/
Alleles
Limits:
Adult
/
Child, preschool
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2013
Document type:
Article