Effect of vasonatrin peptide on the Ca2+ activated K+ channels of vascular smooth muscle cells isolated from rat mesentery arteries / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 94-97, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-254596
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate effect and mechanism of vasonatrin peptide (VNP) on Ca2+ activated K+ channels (K(Ca)) of vascular smooth muscle cells (VSMCs) isolated from rat mesentery arteries.</p><p><b>METHODS</b>Changes of K(Ca) induced by VNP were measured by the means of whole cell recording mode of patch clamp, furthermore effects of HS-142-1(0.3 g/L), 8-Br-cGMP and methylene blue (MB) were observed.</p><p><b>RESULTS</b>K(Ca) was significantly enhanced by VNP (10(-6) mol/L), which was mimicked by 8-Br-cGMP(10(-3) mol/L) and blocked completely by HS-142-1 or MB (2 x 10(-5) mol/L).</p><p><b>CONCLUSION</b>VNP increases K(Ca) of VSMCs isolated from rat mesenteric arteries, by binding with natriuretic peptide guanylate cyclase-coupled receptors and increasing the intracellular level of cGMP in VSMCs.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
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Atrial Natriuretic Factor
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Rats, Sprague-Dawley
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Cell Biology
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Potassium Channels, Calcium-Activated
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Mesenteric Arteries
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Metabolism
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Muscle, Smooth, Vascular
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2006
Document type:
Article