Relationship between expression of STAT3 gene and proliferation of rat pulmonary arterial smooth muscle cells under hypoxia conditions / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 75-79, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-254601
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To explore the relationship between expression of signal transduction and activators of transcription 3 (STAT3) gene and proliferation of rat pulmonary arterial smooth muscle cells (PASMCs) under hypoxia conditions.</p><p><b>METHODS</b>After primarily cultured rat PASMCs was treated with AG490 and then exposed to hypoxia, the tyrosine-phosphorylated STAT3 protein were detected at 2 h, 6 h, 12 h, 16 h, 24 h of exposure to hypoxia by semi-quantitive RT-PCR (sqRT-PCR) and Western blot respectively. The expression of c-myc mRNA was analyzed by sqRT-PCR. 3H-TdR incorporation was used to detect the cell proliferation.</p><p><b>RESULTS</b>The level of tyrosine-phosphorylated STAT3 increased at 6 h and peaked at 12 h. The expression of c-myc mRNA increased after 2 h of hypoxia and reached maximal level at 4 h, then declined at 6 h and to the basal levels at 12 h. With the prolonging of hypoxia time, 3H-TdR incorporation in PASMC under hypoxia conditions was significantly higher. AG490 inhibited proliferation of PASMCs by preventing STAT3 tyrosine phosphorylation and the expression of c-myc under hypoxia conditions.</p><p><b>CONCLUSION</b>(1) The activation of STAT3 and c-myc gene might play an important role in the early stage of hypoxia-induced PASMCs proliferation. (2) STAT3 upregulated the expression of c-myc during the proliferation of PASMCs induced by hypoxia.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pulmonary Artery
/
Signal Transduction
/
Cell Hypoxia
/
Cells, Cultured
/
Rats, Wistar
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Cell Biology
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Myocytes, Smooth Muscle
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Cell Proliferation
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STAT3 Transcription Factor
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Hypoxia
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2006
Document type:
Article