The influence of acetylcholine on N receptor beta-subunit of the gastric epithelia and the gastric adenocarcinoma cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 457-460, 2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-254632
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To study the influence of acetylcholine (ACh) on nicotinic receptor(N receptor) beta-subunit of the gastric epithelia and the gastric adenocarcinoma cells, and the difference of both cells.</p><p><b>METHODS</b>Immunohistochemistry method was used to examine the number, number density and surface density of N receptor beta-subunit in both cells cultured in vitro.</p><p><b>RESULTS</b>The number and number density of N receptor beta-subunit in the gastric adenocarcinoma cells were much more than that in the gastric epithelia (P < 0.05). But surface density of N receptor beta-subunit in the gastric adenocarcinoma cells were lower than that in the gastric epithelia (P < 0.05). ACh at 10(6) mol/L could increase the number, number density and surface density of N receptor beta-subunit in the gastric epithelia (P < 0.01). The increase effect could not be blocked by atropine. ACh had no effect on N receptor beta-subunit in the gastric adenocarcinoma cells.</p><p><b>CONCLUSION</b>ACh at low concentration initiates N receptor desensitization in the gastric epithelia. ACh has no effect on sensitivity of N receptor beta-subunit in the gastric adenocarcinoma cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Stomach Neoplasms
/
Tumor Cells, Cultured
/
Adenocarcinoma
/
Acetylcholine
/
Cells, Cultured
/
Receptors, Nicotinic
/
Cell Biology
/
Epithelial Cells
/
Gastric Mucosa
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2005
Document type:
Article