In vivo imaging of blood flow using two-photon laser scanning fluorescent microscopy / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 245-248, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-255041
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the three-dimensional distribution of vessels, and to establish a new method for measurement of blood flow velocity in mice cerebral cortex using two-photon laser scanning microscopy and fluorescence probe labeling technique.</p><p><b>METHODS</b>The mouse was made cranial window surgery and injected Texas-Red through tail vein after anesthetized. The three-dimensional imaging of vessel was obtained through z-stack scanning, and blood flow velocity was quantified through line scanning.</p><p><b>RESULTS</b>We could detect vascular distribution for more than 500 µm depth using two-photon microscopy. The velocity of blood flow was (0.59 ± 0.12) mm/s in capillary.</p><p><b>CONCLUSION</b>The method for observing the brain blood flow by two-photon microscopy was established, which could achieve quantification of single vascular blood flow velocity and provide experimental evidence for basic research and medical applications.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Blood Flow Velocity
/
Brain
/
Capillaries
/
Cerebrovascular Circulation
/
Fluorescent Dyes
/
Hemodynamics
/
Microscopy, Fluorescence
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2015
Document type:
Article