Establishment of chondrocyte degeneration model in vitro by rat serum / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 308-314, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-255194
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a model of chondrocyte degeneration in vitro.</p><p><b>METHODS</b>Chondrocytes were isolated from articular cartilages of newly born SD rats by digestion with typeⅡ collagenase. The chondrocytes were cultured with H-DMEM medium containing 10%FBS, 50 ng/mL IL-1β+10%FBS, 2.5% rat serum and 5% rat serum, respectively; and the chondrocytes at passage one were used in the experiments. The morphology changes were investigated under phase contrast microscope after chondrocytes were treated with rat serum and IL-1β. Proliferation of chondrocytes was detected by MTT method. The protein expression levels of PCNA, typeⅡ collagen and MMP-13 were examined by Western blotting. The levels of ADAMTS5, MMP-9, Aggrecan and SOX-9 mRNA were detected by real-time PCR.</p><p><b>RESULTS</b>The cell morphology was changed from polygon to spindle in both rat serum groups and IL-1β group, and the proliferation of chondrocytes in these groups was much higher than that in control group. The results showed that the expression levels of typeⅡ collagen, Aggrecan and SOX-9 decreased while the expression levels of MMP-13, MMP-9 and ADMATS5 were up-regulated in rat serum and IL-1β-treated groups compared with control group.</p><p><b>CONCLUSION</b>The results indicate that rat serum can induce chondrocyte degeneration and may be used for osteoarthritis model in vitro.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Pathology
/
Pharmacology
/
RNA, Messenger
/
Cartilage, Articular
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Up-Regulation
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Cells, Cultured
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Rats, Sprague-Dawley
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Proliferating Cell Nuclear Antigen
/
Chondrocytes
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2015
Document type:
Article