Functional changes of dendritic cells after infection by recombinant retrovirus carrying human telomerase reverse transcriptase gene fragment / 南方医科大学学报
Journal of Southern Medical University
; (12): 394-397, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-255302
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the functional changes of dendritic cells (DCs) after infection by recombinant retrovirus carrying human telomerase reverse transcriptase (hTERT) gene fragment.</p><p><b>METHODS</b>Interleukin-12 (IL-12) levels in DC culture supernatant was determined by enzyme-linked immunosorbent assay (ELISA). The abilities of DCs infected with recombinant retrovirus carrying hTERT gene (hTERT-DCs) and non-infected DCs (N-DCs) to stimulate allogeneic lymphocyte proliferation were evaluated with mixed leukocytes reaction (MLR), and the surface markers of DCs including CD80, CD83, CD86 and HLA-DR were detected by flow cytometry. Cytotoxic T lymphocyte (CTL) assay was performed with CytoTox 96 non-radioactive cytoxicity assay.</p><p><b>RESULTS</b>Compared with N-DCs, hTERT-DCs showed no significant changes in IL-12 secretion and capacity to stimulate allogeneic lymphocytes reaction, but had significantly lower CD83 expression. Specific CTLs induced by hTERT-DCs resulted in higher cytotoxicity against telomerase-positive target cells than that against the negative target cells.</p><p><b>CONCLUSION</b>Infection with the recombinant retrovirus carrying hTERT fragment may jeopardize the maturation of DCs, which, however, still retain their capacity to activate and stimulate lymphocyte proliferation and to prime autologous T lymphocytes to generate specific CTL against hTERT.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Recombination, Genetic
/
Retroviridae
/
Virology
/
Dendritic Cells
/
T-Lymphocytes, Cytotoxic
/
Cells, Cultured
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Interleukin-12
/
Telomerase
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Cell Biology
/
Allergy and Immunology
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2006
Document type:
Article