Role and mechanism of all-trans retinoic acid in up-regulating apelin expression in vascular smooth muscle cells / 生理学报
Acta Physiologica Sinica
; (6): 186-192, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-255958
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the mechanism of all-trans retinoic acid (ATRA) up-regulating apelin expression in vascular smooth muscle cells (VSMCs). The effect of ATRA on apelin expression in the VSMCs was investigated by RT-PCR, real-time PCR and Western blot analysis. To further define whether retinoic acid receptor α (RARα) mediated the induction of apelin by ATRA, endogenous RARα was down regulated by transfection of siRNA against RARα (si-RARα) or RARα was over-expressed by infection of the adenovirus vector pAd-GFP-RARα in the VSMCs. The results showed that ATRA significantly induced apelin expression in a time- and dose-dependent manner in the VSMCs. Although RARα expression was increased in a time-dependent manner, the expressions of RARβ and RARγ were little changed by the ATRA treatment. When VSMCs were treated with a RARα antagonist Ro 41-5253 prior to the addition of ATRA, or si-RARα was used to down regulate endogenous RARα expression, the blockade of RARα signaling partially reduced the response of apelin to ATRA. Moreover, RARα over-expression, induced by infection of pAd-GFP-RARα, further increased the induction of apelin by ATRA. In conclusion, ATRA may up-regulate apelin expression in VSMCs, and the mechanism may be RARα dependent.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Tretinoin
/
Benzoates
/
Transfection
/
Signal Transduction
/
Up-Regulation
/
Gene Expression Regulation
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Chromans
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Receptors, Retinoic Acid
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Cell Biology
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Myocytes, Smooth Muscle
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2015
Document type:
Article