Construction of cell factories for high production of nerolidol in Saccharomyces cerevisiae / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2962-2968, 2017.
Article
in Zh
| WPRIM
| ID: wpr-256007
Responsible library:
WPRO
ABSTRACT
Nerolidol is an important constituent of terpenoid essential oil and has excellent anti-tumor, anti-bacterial, and anti-oxidative properties. For realizing heterogenous production of nerolidol, our research firstly integrated the codon-optimized Actinidia sinensis nerolidol synthase gene (NES) into the terpenoid chassis strain FPP-001, and obtained NES-001 that could produce 2.71 mg•L⁻¹ nerolidol. Then, the N-terminal of the NES was fused with FPS by linker peptide GGGS. With this strategy, nerolidol production improved by 59.80-fold, reaching 162.07 mg•L⁻¹. Finally, by introduction of auxotrophic marker TRP1 in NES-002, the resulting strain NES-003 could produce 1 711.53 mg•L⁻¹ by high cell density fermentation method. This study provides the basis for the fermentative production of nerolidol and other sesquiterpenoids.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2017
Document type:
Article