Expression of porcine interferon-gamma gene in Pichia pastoris and its effect of inhibiting porcine reproductive and respiratory syndrome virus / 生物工程学报
Chinese Journal of Biotechnology
; (12): 683-686, 2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-256138
Responsible library:
WPRO
ABSTRACT
In order to develop recombinant porcine Interferon-gamma (rPoIFN-gamma) to prevent porcine viral infection, PoIFN-gamma cDNA lacking the signal peptide was expressed in Pichia pastoris GS115 strain, and the effect of rPoIFN-gamma on porcine reproductive and respiratory syndrome virus (PRRSV) was investigated. The PoIFN-gamma gene was inserted into integrative vector pHIL-S1, and the recombinant GS115 strain (pHIL-S1/PoIFN-gamma) was constructed by homologues recombinant. The rPoIFN-gamma protein was 18 kD with an expressing yield of 18% was assayed by SDS-PAGE and Western blot, respectively. The anti-viral activity of rPoIFN-gamma was in the range of 450-540 u/mL. In addition, the effect of rPoIFN-gamma ant-PRRSV was determined using CPE50 method. The results indicated that high concentration of rPoIFN-gamma could inhibit PRRSV on Marc-145 cell line. The rPoIFN-gamma is a potential drug for prevention and treatment of various kinds of viral pig diseases.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Leprosy
Database:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Pharmacology
/
Pichia
/
Swine
/
Recombinant Proteins
/
Interferon-gamma
/
Porcine respiratory and reproductive syndrome virus
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2002
Document type:
Article