DNA microarray analysis of protective mechanism of buyang huanwu decoction on focal cerebral ischemia/reperfusion in rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 559-563, 2004.
Article
in Zh
| WPRIM
| ID: wpr-256307
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyse the effects of buyang huanwu decoction (BYHWT) on differentially expressed genes during cerebral ischemia/reperfusion in rats with DNA microarray.</p><p><b>METHOD</b>cDNA microarray chips containing 512 cDNAs were made by Biostar Genechip Inc. Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) with an filament. Saline or BYHWT was given p.o. after onset of cerebral ischemia and brains were removed after 24 h of recirculation for mRNAs isolation. A differential measurment of mRNAs from post-ischemic and BYHWT treated animals was performed with microarray.</p><p><b>RESULT</b>Up-and down-regulated genes were 69 and 80 in ischemic group. Up-and down-regulated genes were 25 and 6 in BYHWT treated group.</p><p><b>CONCLUSION</b>BYHWT regulates the differential expression genes after focal brain ischemia/reperfusion in rats, due to its mechanism of protecting cerebral ischemia/reperfusion injury.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Drugs, Chinese Herbal
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Reperfusion Injury
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Brain Ischemia
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Rats, Sprague-Dawley
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Neuroprotective Agents
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Oligonucleotide Array Sequence Analysis
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Gene Expression Profiling
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Genetics
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2004
Document type:
Article