Effect of puerarin and daidzein on proliferating vascular smooth muscle cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 437-440, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-256333
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the different effects of Puerarin and Daidzein on the expression of proliferating vascular smooth muscle cells, and to discuss the mechanism.</p><p><b>METHOD</b>MT was used to detect the state of VSMC (vascular smooth muscle cell) activity. The expression levels of Survivin, Bcl-xl, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) were analyzed quantitatively by reverse transcriptase polymerase chain reaction (Rt-PCR).</p><p><b>RESULT</b>Compared with Puerarin groups, VSMC activity in daidzein groups was lower, and the ratio of Bax/Gapdh/Bcl-xl/Gapdh was higher.</p><p><b>CONCLUSION</b>The inhibition effect of daidzein on VSMC proliferation is stronger than that of puerarin.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Vasodilator Agents
/
RNA, Messenger
/
Cells, Cultured
/
Chemistry
/
Gene Expression Regulation
/
Proto-Oncogene Proteins c-bcl-2
/
Pueraria
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2004
Document type:
Article