The fibroblast primary cell culture by the split-thickness skin slide technique / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 450-451, 2003.
Article
in Zh
| WPRIM
| ID: wpr-256412
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To acquire lots of cell to culture during the primary cell culture.</p><p><b>METHOD</b>We take the split-thickness skin slide technique to acquire the dissociated fibroblast cell in two big-ear rats.</p><p><b>RESULTS</b>The cell number is above 10(6) from 1 cm x 2 cm split-thickness skin slide and the technique is simple, economic, effectve.</p><p><b>CONCLUSION</b>We think this way is better than other methods, and should be adopted in the primary cell culture, especially in fibroblast transplantation by injection.</p>
Full text:
1
Database:
WPRIM
Main subject:
Transplantation
/
Cell Culture Techniques
/
Cell Biology
/
Fibroblasts
/
Methods
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Plastic Surgery
Year:
2003
Document type:
Article