Application of reverse transcriptase polymerase chain reaction for surveillance of influenza virus in Beijing / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 382-385, 2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-257245
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the RT-PCR method to type the influenza virus.</p><p><b>METHODS</b>After amplifying the virus by cell culture, we carried out RT-PCR by using two pairs typing primers and four pairs subtyping primers to detect the influenza virus.</p><p><b>RESULTS</b>In those 23 samples which had cytopathologic changes, there were 10 positive strains detected by RT-PCR assay including seven A type (six H3N2 subtype and one H1N1 subtype) and three B type.</p><p><b>CONCLUSION</b>This method is rapid, specific and sensitive and possesses great value for practical application in the surveillance of influenza virus.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Orthomyxoviridae
/
Sensitivity and Specificity
/
Classification
/
Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2002
Document type:
Article