Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 440-445, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-257614
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice.</p><p><b>METHODS</b>A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each) the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery.</p><p><b>RESULT</b>The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05).</p><p><b>CONCLUSION</b>The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Wounds and Injuries
/
Enzyme-Linked Immunosorbent Assay
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Reperfusion Injury
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Chemokines
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Intercellular Signaling Peptides and Proteins
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Intestines
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Ischemia
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Mice, Inbred C57BL
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2015
Document type:
Article