The effects of sera from patients with paroxysmal nocturnal hematoglobinuria on the growth of CD34(+) cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 47-51, 2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-258071
Responsible library:
WPRO
ABSTRACT
To investigate the effects of sera on the growth of single CD34(+) cells from patients with paroxysmal nocturnal hematoglobinuria (PNH), sera from both PNH patients and normal individuals were added separately to the single cell culture system and semi-solid colony formation system. The growth of the normal CD34(+) and PNH CD34(+) CD59(+) and CD34(+) CD59(-) cells was evaluated. No growth difference was found for growth of the normal CD34(+) and PNH CD34(+) CD59(+) cells when PNH or normal sera were added to the culture media in either single cell culture or colony formation culture. While no difference was detected for single PNH CD34(+) CD59(-) cells growth when PNH or normal sera were added, more colonies were observed in semi-solid culture with PNH sera. A conclusion was reached that compared with those from normal controls, the sera from PNH patients had no significant influence on single hematopoietic stem/progenitor cells derived from normal subjects and from PNH patients, but the PNH sera might promote the colony formation of the CD34(+) CD59(+) cells in semi-solid culture
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Hematopoietic Stem Cells
/
Blood Proteins
/
Cell Division
/
Cells, Cultured
/
CD59 Antigens
/
Antigens, CD34
/
Allergy and Immunology
/
Hemoglobinuria, Paroxysmal
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2002
Document type:
Article